Yang J, Yi P, Wei L, Xu Z, Chen Y, Tang L et al. Analysis of the CDR3 length of TCR alphabeta T cells in the peripheral blood of patients with chronic hepatitis B. Yao XS, Zhang GW, Ma L, Wen Q, Hou JL, Meng MJ et al. Effect of telbivudine therapy on the cellular immune response in chronic hepatitis B. Hepatology 2007 45: 507–539.Ĭhen Y, Li X, Ye B, Yang X, Wu W, Chen B et al. Zhong hua Gan Zang Bing Za Zhi 2000 8: 324–329. Management scheme of diagnostic and therapy criteria of viral hepatitis. Cancer Epidemiol 2010 34: 733–740.Ĭhinese Society of Infectious Diseases and Parasitic Diseases CSoH, Chinese Medical Association. Primary exploration of CDR3 spectratyping and molecular features of TCR beta chain in the peripheral blood and tissue of patients with colorectal carcinoma. Zhou J, Ma R, Luo R, Sun Y, He X, Sun W et al. Rapid detection of clonal expansion of T-cell receptor-beta gene in patients with HBV using the real-time PCR with DNA melting curve analysis. Yang JZ, Li MW, Wang JG, Lu HF, Yao XS, He JQ et al. The polyclonal CD8 T cell response to influenza M158-66 generates a fully connected network of cross-reactive clonotypes to structurally related peptides: a paradigm for memory repertoire coverage of novel epitopes or escape mutants. Increased levels of arginase in patients with acute hepatitis B suppress antiviral T cells. Sandalova E, Laccabue D, Boni C, Watanabe T, Tan A, Zong HZ et al. Rapid T cell receptor delineation reveals clonal expansion limitation of the magnitude of the HIV-1-specific CD8 + T cell response. Gut 2012 61 Suppl 1: i6–i17.īalamurugan A, Ng HL, Yang OO. New insight in the pathobiology of hepatitis B virus infection. Effect of age on the incidence of acute hepatitis B after 25 years of a universal newborn hepatitis B immunization program in Taiwan. Su WJ, Liu CC, Liu DP, Chen SF, Huang JJ, Chan TC et al. The natural history of chronic hepatitis B virus infection. Treatment of chronic hepatitis B virus infection in resource-constrained settings: expert panel consensus. Wiersma ST, McMahon B, Pawlotsky JM, Thio CL, Thursz M, Lim SG et al. The preferentially selected TRBV5.1 and BV20 with the relatively conserved CDR3 motif may be potential targets for personalized treatments of chronic HBV infection. TRBV11, BV15 and BV20, especially from the CD8 + T-cell subset, may be relevant to the pathogenesis of subjects with AHI. These results demonstrate the presence of multiple biased TRBV families in recovered AHI subjects.
The relatively conserved amino acid motifs of TRBV5.1 and BV20 CDR3 were also detected in the CD4 + and CD8 + T-cell subsets. Compared to other members of the TRBV gene family, TRBV11, BV15 and BV20 were predominantly expressed in the repertoire of peripheral blood lymphocytes in recovered AHI subjects. The frequency of monoclonally expanded TRBV genes in the CD8 + T-cell subset was significantly higher than that of the CD4 + T-cell subset and the PBMCs. Skewed expansions of multiple TRBV genes were observed among the CD4 + and CD8 + T-cell subsets and the PBMCs. When a GMSP profile showed a single peak, the monoclonally expanded TRBV gene was cloned and sequenced. The molecular features of the TRBV complementary determining region 3 (CDR3) motifs were determined using GMSP analysis. To explore the portrait of the TRBV family in peripheral blood lymphocytes from subjects who have recovered from acute hepatitis B virus infection (AHI), peripheral blood mononuclear cells (PBMCs) were separated and further sorted into CD4 + and CD8 + T-cell subsets. The gene melting spectral pattern (GMSP) can be used to determine the profile of the TRBV gene family. The profile of T-cell receptor beta-chain variable (TRBV) genes usually skews in subjects with virus infection or cancer.
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